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longipalpis feeding sources, plant DNA was not detected in nearly half of the caught sand flies.This may be explained by factors such as DNA degradation by enzymes in the digestive tract—a process that occurs with blood DNA ingested by females during blood feeding.Of further note, some plant families (i.e., Rutacea and Annonacea) with abundance higher than that of Fabaceae were found in none of the sand flies (Table 2). The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.This attests to the fact that sand flies have a feeding preference for certain plants or plant families. The use of the DNA barcode tool, a technique that is able to identify the food content in invertebrates, using short sequences obtained in conserved regions of the chloroplast, has been widely reported in the literature to identify plant-derived food content in wild-caught Phlebotomus papatasi sand flies.The DNA barcode approach can be used to identify relationships between presence of plant types and vectorial capacity or simply the likelihood of vector habitats in a particular area.At the sand fly trapping site, plant species belonging to 14 families were collected and identified.The Fabaceae family was the most frequently detected food source of L.
This study verified the feasibility of detecting plant DNA in the digestive tracts of L. The successful application of this approach is consistent with previous studies investigating feeding behavior of P. However, despite the success of the approach presented here in distinguishing L.
longipalpis captured during 5 days in the tropical urban environment of Teresina, Piauí State.
Fifty-seven percent of the flies were found positive for plant DNA.
The DNA barcode technique was applied to identify plant food source of wild-caught L.
longipalpis using specific primers for a locus from the chloroplast genome, ribulose diphosphate carboxylase.